Design Methodology of a New Wavelet Basis Function for Fetal Phonocardiographic Signals

Fetal phonocardiography (fPCG) based antenatal care system is economical and has a potential to use for long-term monitoring due to noninvasive nature of the system. The main limitation of this technique is that noise gets superimposed on the useful signal during its acquisition and transmission. Conventional filtering may result into loss of valuable diagnostic information from these signals. This calls for a robust, versatile, and adaptable denoising method applicable in different operative circumstances. In this work, a novel algorithm based on wavelet transform has been developed for denoising of fPCG signals. Successful implementation of wavelet theory in denoising is heavily dependent on selection of suitable wavelet basis function. This work introduces a new mother wavelet basis function for denoising of fPCG signals. The performance of newly developed wavelet is found to be better when compared with the existing wavelets. For this purpose, a two-channel filter bank, based on characteristics of fPCG signal, is designed. The resultant denoised fPCG signals retain the important diagnostic information contained in the original fPCG signal

Cyclodextrin Complexes of Reduced Bromonoscapine in Guar Gum Microspheres Enhance Colonic Drug Delivery

Here, we report improved solubility and enhanced colonic delivery of reduced bromonoscapine (Red-Br-Nos), a cyclic ether brominated analogue of noscapine, upon encapsulation of its cyclodextrin (CD) complexes in bioresponsive guar gum microspheres (GGM). Phase−solubility analysis suggested that Red-Br-Nos complexed with β-CD and methyl-β-CD in a 1:1 stoichiometry, with a stability constant (Kc) of 2.29 × 103 M−1 and 4.27 × 103 M−1. Fourier transforms infrared spectroscopy indicated entrance of an O−CH2 or OCH3−C6H4−OCH3 moiety of Red-Br-Nos in the β-CD or methyl-β- CD cavity. Furthermore, the cage complex of Red-Br-Nos with β-CD and methyl-β-CD was validated by several spectral techniques. Rotating frame Overhauser enhancement spectroscopy revealed that the Ha proton of the OCH3−C6H4−OCH3 moiety was closer to the H5 proton of β-CD and the H3 proton of the methyl-β-CD cavity. The solubility of Red-Br-Nos in phosphate buffer saline (PBS, pH ∼ 7.4) was improved by ∼10.7-fold and ∼21.2-fold when mixed with β-CD and methyl-β-CD, respectively. This increase in solubility led to a favorable decline in the IC50 by ∼2-fold and ∼3-fold for Red-Br-Nos−β-CD-GGM and Red-Br-Nos−methyl-β-CD-GGM formulations respectively, compared to free Red-Br-Nos−β-CD and Red-Br-Nos−methyl-β-CD in human colon HT-29 cells. GGM-bearing drug complex formulations were found to be highly cytotoxic to the HT-29 cell line and further effective with simultaneous continuous release of Red-Br-Nos from microspheres. This is the first study to showing the preparation of drug-complex loaded GGMS for colon delivery of Red-Br-Nos that warrants preclinical assessment for the effective management of colon cancer